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J Obstet Gynaecol Res. 2017 Mar;43(3):571-579. doi: 10.1111/jog.13233. Epub 2017 Feb 4.

Macrophage migration inhibitory factor promoter polymorphisms (-794CATT5-7 ) as potential biomarker for early-stage cervical cancer.

Author information

1
Department of Obstetrics and Gynecology, Shanxi Academy of Medical Sciences & Shanxi Dayi Hospital, Dayi Hospital of Shanxi Medical University, Taiyuan, China.
2
Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, China.

Abstract

AIM:

The objective was to investigate the correlation between macrophage migration inhibitory factor (MIF) promoter polymorphisms (-794CATT5-7 ) and early-stage cervical cancer (ESCC) and to identify a potential biomarker for ESCC.

METHODS:

A hospital-based case-control study was performed. The case group contained 250 patients with histologically confirmed ESCC. The control group included 147 healthy women. Polymerase chain reaction-single strand conformation polymorphism was used to genotype polymorphisms of MIF promoter -794CATT5-7 . Enzyme-linked immunosorbent assay was applied to detect serum concentration of MIF.

RESULTS:

The genotype distribution and allele frequency of MIF-794CATT in the ESCC group were significantly different from those in the control group (P < 0.05). The 7-CATT repeat carriers were significantly higher in the ESCC group than those in the control group (P < 0.05). The 7-CATT repeat carriers (5/7, 6/7, and 7/7) were associated with ESCC and increased risks of cervical cancer (odds ratio = 3.5, 3.0, and 5.6; 95% confidence interval = 1.2-10.5, 1.2-7.9, and 1.3-25.3, respectively). Serum concentration of MIF was significantly higher in the ESCC group than in the control group (P < 0.05), and it was significantly higher in 7-CATT carriers than in non-7-CATT carriers (P < 0.05). Neither polymorphisms of MIF-794 nor serum MIF were associated with lymph node metastases and differentiation (P > 0.05).

CONCLUSION:

MIF promoter polymorphisms (-794CATT) were correlated with ESCC; and 7-CATT might play a role in ESCC. It could be a potential biomarker for ESCC.

KEYWORDS:

biomarker; cervical cancer; lymph node metastases; macrophage migration inhibitory factor; microsatellite polymorphisms

PMID:
28160516
DOI:
10.1111/jog.13233
[Indexed for MEDLINE]

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