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Plant Physiol. 2017 Mar;173(3):1811-1823. doi: 10.1104/pp.16.01959. Epub 2017 Feb 3.

Physiological Responses and Gene Co-Expression Network of Mycorrhizal Roots under K+ Deprivation.

Garcia K1,2,3, Chasman D1,2,3, Roy S1,2,3, Ané JM4,5,6.

Author information

1
Department of Bacteriology (K.G., J.-M.A.), Department of Computer Sciences (S.R.), and Department of Agronomy (J.-M.A.), University of Wisconsin, Madison, Wisconsin 53706.
2
Wisconsin Institute for Discovery, University of Wisconsin, Madison, Wisconsin 53715 (D.C., S.R.); and.
3
Department of Biostatistics and Medical Informatics, University of Wisconsin, Madison, Wisconsin 53792 (S.R.).
4
Department of Bacteriology (K.G., J.-M.A.), Department of Computer Sciences (S.R.), and Department of Agronomy (J.-M.A.), University of Wisconsin, Madison, Wisconsin 53706; jeanmichel.ane@wisc.edu.
5
Wisconsin Institute for Discovery, University of Wisconsin, Madison, Wisconsin 53715 (D.C., S.R.); and jeanmichel.ane@wisc.edu.
6
Department of Biostatistics and Medical Informatics, University of Wisconsin, Madison, Wisconsin 53792 (S.R.) jeanmichel.ane@wisc.edu.

Abstract

Arbuscular mycorrhizal (AM) associations enhance the phosphorous and nitrogen nutrition of host plants, but little is known about their role in potassium (K+) nutrition. Medicago truncatula plants were cocultured with the AM fungus Rhizophagus irregularis under high and low K+ regimes for 6 weeks. We determined how K+ deprivation affects plant development and mineral acquisition and how these negative effects are tempered by the AM colonization. The transcriptional response of AM roots under K+ deficiency was analyzed by whole-genome RNA sequencing. K+ deprivation decreased root biomass and external K+ uptake and modulated oxidative stress gene expression in M. truncatula roots. AM colonization induced specific transcriptional responses to K+ deprivation that seem to temper these negative effects. A gene network analysis revealed putative key regulators of these responses. This study confirmed that AM associations provide some tolerance to K+ deprivation to host plants, revealed that AM symbiosis modulates the expression of specific root genes to cope with this nutrient stress, and identified putative regulators participating in these tolerance mechanisms.

PMID:
28159827
PMCID:
PMC5338680
DOI:
10.1104/pp.16.01959
[Indexed for MEDLINE]
Free PMC Article

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