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Int J Infect Dis. 2017 Mar;56:167-175. doi: 10.1016/j.ijid.2017.01.021. Epub 2017 Jan 31.

Humoral immune profiling of mycobacterial antigen recognition in sarcoidosis and Löfgren's syndrome using high-content peptide microarrays.

Author information

1
Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden; Department of Respiratory Medicine and Allergy, Karolinska University Hospital, Solna, Sweden.
2
Centre for Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden; Division of Therapeutic Immunology (TIM), Department of Laboratory Medicine (LABMED), Karolinska Institutet, Huddinge 14186, Stockholm, Sweden.
3
Department of Medicine Solna, Karolinska Institutet, Stockholm, Sweden.
4
Angered's Hospital, Gothenburg, Sweden.
5
Centre for Infectious Medicine (CIM), Department of Medicine (MedH), Karolinska Institutet, Stockholm, Sweden.
6
Division of Therapeutic Immunology (TIM), Department of Laboratory Medicine (LABMED), Karolinska Institutet, Huddinge 14186, Stockholm, Sweden.
7
Centre for Clinical Microbiology, Division of Infection and Immunity, University College London, and NIHR Biomedical Research Centre, UCL Hospitals NHS Foundation Trust, London, UK.
8
Centre for Allogeneic Stem Cell Transplantation (CAST), Karolinska University Hospital, Huddinge, Sweden; Division of Therapeutic Immunology (TIM), Department of Laboratory Medicine (LABMED), Karolinska Institutet, Huddinge 14186, Stockholm, Sweden. Electronic address: markus.maeurer@ki.se.

Abstract

INTRODUCTION:

Sarcoidosis is considered an idiopathic granulomatous disease, although similar immunological and clinical features with tuberculosis (TB) suggest mycobacterial involvement in its pathogenesis. High-content peptide microarrays (HCPM) may help to decipher mycobacteria-specific antibody reactivity in sarcoidosis.

METHODS:

Serum samples from patients with sarcoidosis, Löfgren's syndrome, and TB, as well as from healthy individuals (12/group), were tested on HCPM containing 5964 individual peptides spanning 154 Mycobacterium tuberculosis proteins displayed as 15-amino acid stretches. Inclusion/exclusion and significance analyses were performed according to published methods.

RESULTS:

Each study group recognized 68-78% M. tuberculosis peptides at least once. M. tuberculosis epitope recognition by sarcoidosis patient sera was 42.7%, and by TB patient sera was 39.1%. Seven and 16 peptides were recognized in 9/12 (75%) and 8/12 (67%) sarcoidosis patient sera but not in TB patient sera, respectively. Nine (75%) and eight (67%) out of twelve TB patient sera, respectively recognized M. tuberculosis peptides that were not recognized in sarcoidosis patient sera.

CONCLUSIONS:

Specific IgG recognition patterns for M. tuberculosis antigens in sarcoidosis patients re-affirm mycobacterial involvement in sarcoidosis, providing biologically relevant targets for future studies pertaining to diagnostics and immunotherapy.

KEYWORDS:

Humoral immune responses; Löfgren’s syndrome; Peptide microarray; Sarcoidosis; Tuberculosis; tuberculosis antigens

PMID:
28159576
DOI:
10.1016/j.ijid.2017.01.021
[Indexed for MEDLINE]
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