Format

Send to

Choose Destination
Stem Cell Reports. 2016 Nov 8;7(5):970-982. doi: 10.1016/j.stemcr.2016.10.001. Epub 2016 Oct 27.

High-Throughput Toxicity and Phenotypic Screening of 3D Human Neural Progenitor Cell Cultures on a Microarray Chip Platform.

Author information

1
Chemical and Biological Engineering and Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
2
Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
3
Bioengineering, University of California, Berkeley, CA 94720, USA.
4
Department of Bioengineering and Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisbon 1049-001, Portugal.
5
Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA; Bioengineering, University of California, Berkeley, CA 94720, USA.
6
Chemical and Biological Engineering and Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA. Electronic address: dordick@rpi.edu.

Abstract

A 3D cell culture chip was used for high-throughput screening of a human neural progenitor cell line. The differential toxicity of 24 compounds was determined on undifferentiated and differentiating NPCs. Five compounds led to significant differences in IC50 values between undifferentiated and differentiating cultures. This platform has potential use in phenotypic screening to elucidate molecular toxicology on human stem cells.

KEYWORDS:

high-content screening; high-throughput microarray; human neural stem cells; neurotoxicity; screening; three-dimensional cell culture

PMID:
28157485
PMCID:
PMC5106528
DOI:
10.1016/j.stemcr.2016.10.001
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center