Format

Send to

Choose Destination
Int J Biol Macromol. 2017 May;98:182-187. doi: 10.1016/j.ijbiomac.2017.01.114. Epub 2017 Jan 30.

Extraction and purification of pumpkin polysaccharides and their hypoglycemic effect.

Author information

1
College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang, 310018, China.
2
College of Life Sciences, China JiLiang University, Hangzhou, Zhejiang, 310018, China. Electronic address: yjzhang@vip.163.com.

Abstract

The anti-diabetic activity of the polysaccharides (PPs) obtained from the dried pumpkin pulp was studied in this paper. The PPs were administered by intraperitoneal injection to the alloxan-induced diabetic male ICR mice. The PPs hypoglycemic effect was evaluated by testing the fast blood glucose level, fasting serum insulin and hepatic glycogen. After 7h administration, the PPs showed a significantly hypoglycemic effect (p<0.01) and could significantly increase hepatic glycogen and insulin level (p<0.05). PPs-e with potential to enhance the islet cells proliferation activity by MTT assays in vitro was obtained from the PPs through alcohol fractional precipitation and gel chromatography. PPs-e composed of rhamnose, arabinose, glucose, galactose, and little amount of inositol could maintain the blood glucose at a low level in diabetic mice and could even last for more than 24h. These results suggest the potential hypoglycemic effect of the pumpkin polysaccharides in alloxan-induced diabetic mice and for the treatment of diabetic mellitus.

KEYWORDS:

Anti-diabetic effects; Proliferation activity; Pumpkin polysaccharide

PMID:
28153462
DOI:
10.1016/j.ijbiomac.2017.01.114
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center