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Curr Protoc Immunol. 2017 Feb 2;116:2.3.1-2.3.4. doi: 10.1002/cpim.18.

Double-Immunodiffusion Assay for Detecting Specific Antibodies (Ouchterlony).

Author information

1
Cell Signaling Technologies, Danvers, Massachusetts.

Abstract

The method first described by Ouchterlony in 1948 is a classic and simple technique that permits evaluation and comparison of antibodies in animal or human sera directed against protein or complex carbohydrate antigens. It is a low-tech procedure that may provide information on the relative quantity of antibody activity and the nature of the antigenic epitopes in different preparations.

KEYWORDS:

agar; antibody assay; antigen; double-diffusion; gel

PMID:
28150863
DOI:
10.1002/cpim.18
[Indexed for MEDLINE]

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