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Cold Spring Harb Protoc. 2017 Feb 1;2017(2). doi: 10.1101/pdb.prot094888.

Preparation of Slice Cultures from Rodent Hippocampus.

Author information

1
Institute for Synaptic Physiology, Center for Molecular Neurobiology (ZMNH), 20251 Hamburg, Germany.
2
Institute for Synaptic Physiology, Center for Molecular Neurobiology (ZMNH), 20251 Hamburg, Germany thomas.oertner@zmnh.uni-hamburg.de.

Abstract

This protocol describes the preparation of hippocampal slice cultures from rat or mouse pups using sterile conditions that do not require the use of antibiotics or antimycotics. Combining very good optical and electrophysiological accessibility with a lifetime approaching that of the intact animal, many fundamental questions about synaptic plasticity and long-term dynamics of network connectivity can be addressed with this preparation.

PMID:
28148854
DOI:
10.1101/pdb.prot094888
[Indexed for MEDLINE]

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