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Int J Biol Macromol. 2017 May;98:515-525. doi: 10.1016/j.ijbiomac.2017.01.120. Epub 2017 Jan 29.

Antimicrobial and anticancer activities of porous chitosan-alginate biosynthesized silver nanoparticles.

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Division of Bioengineering, Incheon National University, Incheon 406-772, Republic of Korea.
Institute for Life Science of Seogo (ILSS), Kolmar Korea Co, Seoul 137-876, Republic of Korea.
Department of Preventive Dental Sciences, College of Dentistry, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
Division of Bioengineering, Incheon National University, Incheon 406-772, Republic of Korea. Electronic address:
Specialized Graduate School Science & Technology Convergence, Pukyong National University, Busan 608-739, Republic of Korea. Electronic address:


The main aim of this study was to obtain porous antimicrobial composites consisting of chitosan, alginate, and biosynthesized silver nanoparticles (AgNPs). Chitosan and alginate were used owing to their pore-forming capacity, while AgNPs were used for their antimicrobial property. The developed porous composites of chitosan-alginate-AgNPs were characterized using Fourier transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy, X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). The FT-IR results revealed the presence of a strong chemical interaction between chitosan and alginate due to polyelectrolyte complex; whereas, the XRD results confirmed the presence of AgNPs in the composites. The dispersion of AgNPs in the porous membrane was uniform with a pore size of 50-500μm. Antimicrobial activity of the composites was checked with Escherichia coli and Staphylococcus aureus. The developed composites resulted in the formation of a zone of inhibition of 11±1mm for the Escherichia coli, and 10±1mm for Staphylococcus aureus. The bacterial filtration efficiency of chitosan-alginate-AgNPs was 1.5-times higher than that of the chitosan-alginate composite. The breast cancer cell line MDA-MB-231 was used to test the anticancer activity of the composites. The IC50 value of chitosan-alginate-AgNPs on MDA-MB-231 was 4.6mg. The developed chitosan-alginate-AgNPs composite showed a huge potential for its applications in antimicrobial filtration and cancer treatment.


Alginate; Antimicrobial; Chitosan; Composites; Silver nanoparticles

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