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Invest Ophthalmol Vis Sci. 2017 Feb 1;58(2):708-719. doi: 10.1167/iovs.16-21060.

Subretinal Pigment Epithelial Deposition of Drusen Components Including Hydroxyapatite in a Primary Cell Culture Model.

Author information

1
UCL Institute of Ophthalmology, University College London, London, United Kingdom 2Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, London, United Kingdom.
2
UCL Institute of Ophthalmology, University College London, London, United Kingdom 3Centre for Experimental Medicine, School of Medicine, Dentistry and Biomedical Science, Queen's University Belfast, Belfast, Northern Ireland, United Kingdom.
3
Center for Advanced Radiation Sources, The University of Chicago, Chicago, Illinois, United States.
4
Department of Materials, Imperial College London, London, United Kingdom.
5
Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, London, United Kingdom.
6
Department of Ophthalmology, University of Alabama School of Medicine, Birmingham, Alabama, United States.

Abstract

Purpose:

Extracellular deposits containing hydroxyapatite, lipids, proteins, and trace metals that form between the basal lamina of the RPE and the inner collagenous layer of Bruch's membrane are hallmarks of early AMD. We examined whether cultured RPE cells could produce extracellular deposits containing all of these molecular components.

Methods:

Retinal pigment epithelium cells isolated from freshly enucleated porcine eyes were cultured on Transwell membranes for up to 6 months. Deposit composition and structure were characterized using light, fluorescence, and electron microscopy; synchrotron x-ray diffraction and x-ray fluorescence; secondary ion mass spectroscopy; and immunohistochemistry.

Results:

Apparently functional primary RPE cells, when cultured on 10-μm-thick inserts with 0.4-μm-diameter pores, can produce sub-RPE deposits that contain hydroxyapatite, lipids, proteins, and trace elements, without outer segment supplementation, by 12 weeks.

Conclusions:

The data suggest that sub-RPE deposit formation is initiated, and probably regulated, by the RPE, as well as the loss of permeability of the Bruch's membrane and choriocapillaris complex associated with age and early AMD. This cell culture model of early AMD lesions provides a novel system for testing new therapeutic interventions against sub-RPE deposit formation, an event occurring well in advance of the onset of vision loss.

PMID:
28146236
PMCID:
PMC5295770
DOI:
10.1167/iovs.16-21060
[Indexed for MEDLINE]
Free PMC Article

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