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Br J Dermatol. 2017 Sep;177(3):801-808. doi: 10.1111/bjd.15362. Epub 2017 Jun 12.

Multiple variants in 5q31.1 are associated with systemic lupus erythematosus susceptibility and subphenotypes in the Han Chinese population.

Wen LL1,2, Zhu ZW1,2, Yang C1,2, Liu L1,2, Zuo XB1,2, Morris DL3, Dou JF1,2, Ye L1,2, Cheng YY1,2, Guo HM1,2, Huang HQ1,2, Lin Y1,2,4, Zhu CH1,2, Tang LL1,2, Chen MY1,2, Zhou Y1,2, Ding YT1,2, Liang B1,2, Zhou FS1,2, Gao JP1,2, Tang XF1,2, Zheng XD1,2, Wang WJ1,2, Yin XY5, Tang HY1,2, Sun LD1,2, Yang S1,2, Zhang XJ1,2, Sheng YJ1,2, Cui Y6.

Author information

1
Institute of Dermatology and Department of Dermatology, the First Affiliated Hospital, Anhui Medical University, 81 Meishan Road, Hefei, Anhui, 230032, China.
2
Key Laboratory of Dermatology, Anhui Medical University, Ministry of Education, China, Hefei, Anhui, 230032, China.
3
Division of Genetics and Molecular Medicine, King's College London, U.K.
4
Department of Dermatology, the Fourth Affiliated Hospital, Anhui Medical University, Hefei, Anhui, 230032, China.
5
Department of Genetics, and Renaissance Computing Institute, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, U.S.A.
6
Department of Dermatology, China-Japan Friendship Hospital, East Street Cherry Park, Chaoyang District, Beijing, 100029, China.

Abstract

BACKGROUND:

A previous study provided evidence for a genetic association between PPP2CA on 5q31.1 and systemic lupus erythematosus (SLE) across multi-ancestral cohorts, but failed to find significant evidence for an association in the Han Chinese population.

OBJECTIVES:

To explore the association between this locus and SLE using data from our previously published genome-wide association study (GWAS).

METHODS:

Single-nucleotide polymorphisms (SNPs) rs7726414 and rs244689 (near TCF7 and PPP2CA in 5q31.1) were selected as candidate independent associations from a large-scale study in a Han Chinese population consisting of 1047 cases and 1205 controls. Subsequently, 3509 cases and 8246 controls were genotyped in two further replication studies. We then investigated the SNPs' associations with SLE subphenotypes and gene expression in peripheral blood mononuclear cells.

RESULTS:

Highly significant associations with SLE in the Han Chinese population were detected for SNPs rs7726414 and rs244689 by combining the genotype data from our previous GWAS and two independent replication cohorts. Further conditional analyses indicated that these two SNPs contribute to disease susceptibility independently. A significant association with SLE, age at diagnosis < 20 years, was found for rs7726414 (P = 0·001). The expression levels of TCF7 and PPP2CA messenger RNA in patients with SLE were significantly decreased compared with those in healthy controls.

CONCLUSIONS:

This study found evidence for multiple associations with SLE in 5q31.1 at genome-wide levels of significance for the first time in a Han Chinese population, in a combined genotype dataset. These findings suggest that variants in the 5q31.1 locus not only provide novel insights into the genetic architecture of SLE, but also contribute to the complex subphenotypes of SLE.

PMID:
28144936
DOI:
10.1111/bjd.15362
[Indexed for MEDLINE]

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