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Cytokine. 2017 Apr;92:103-109. doi: 10.1016/j.cyto.2017.01.014. Epub 2017 Jan 29.

Homeopathic medicine Cantharis modulates uropathogenic E. coli (UPEC)-induced cystitis in susceptible mice.

Author information

1
Paulista University (UNIP), Research Center, Av José Maria Whitaker, 290, São Paulo, Brazil; University of Santo Amaro (UNISA), Rua Professor Enéas de Siqueira Neto, 340, São Paulo, Brazil.
2
Paulista University (UNIP), Research Center, Av José Maria Whitaker, 290, São Paulo, Brazil.
3
Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas (UNICAMP), Piracicaba, Brazil.
4
Faculty of Veterinary Medicine, University of São Paulo (USP), Av. Prof. Dr. Orlando Marques de Paiva, 87-Cidade Universitária, São Paulo, Brazil.
5
Paulista University (UNIP), Research Center, Av José Maria Whitaker, 290, São Paulo, Brazil. Electronic address: leonibonamin@unip.br.

Abstract

OBJECTIVE:

This is a random blinded placebo controlled murine experimental model to study the effects of Cantharis 6 CH, a homeopathic medicine, on E coli-induced cystitis.

METHODS:

24 adult susceptible female BALB/c mice were inoculated with E coli - UPEC O4:K-:H5 by a transurethral catheter. Cantharis 6cH or vehicle (placebo) was offered to mice by free access into the drinking water (1:100), during 24 h after infection. Spleen, bladder and kidneys were processed for quantitative histopathology after immunohistochemistry, using anti-CD3, CD79, MIF, NK and VEGF antibodies; the cytokines present in the bladder washing fluid were measured using a LUMINEX-Magpix KIT. Mann-Whitney and Fisher exact test were used as statistical analysis.

RESULTS:

Cantharis 6 CH increased IL12p40, IFN-γ and decreased IL10 concentrations in the bladder fluid (p⩽0.05); in the bladder mucosa, it increased the ratio between B and T lymphocytes (31%) and between B lymphocytes and MIF+ macrophages (57%, p⩽0.05). In the pelvis, instead, it decreased the B/T cells ratio (41%, p⩽0.05) and increased the M1/M2 macrophage ratio (42%, p⩽0.05). No differences were seen in the kidney and spleen analysis.

CONCLUSION:

The inverted balance of inflammatory cells and cytokines in bladder and pelvis mucosa shows specific local immune modulation induced by Cantharis 6cH.

KEYWORDS:

Cantharis; Cytokines; Escherichia coli; Homeopathy; UPEC

PMID:
28142108
DOI:
10.1016/j.cyto.2017.01.014
[Indexed for MEDLINE]

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