Format

Send to

Choose Destination
Sci Rep. 2017 Jan 31;7:41528. doi: 10.1038/srep41528.

The hypothalamic NPVF circuit modulates ventral raphe activity during nociception.

Author information

1
Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA.
2
Department of Bioengineering and CNC program, Stanford University, Stanford, CA 94305, USA.
3
Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA.
4
INSERM U1024, Ecole Normale Supérieure Paris, 75005, France.

Abstract

RFamide neuropeptide VF (NPVF) is expressed by neurons in the hypothalamus and has been implicated in nociception, but the circuit mechanisms remain unexplored. Here, we studied the structural and functional connections from NPVF neurons to downstream targets in the context of nociception, using novel transgenic lines, optogenetics, and calcium imaging in behaving larval zebrafish. We found a specific projection from NPVF neurons to serotonergic neurons in the ventral raphe nucleus (vRN). We showed NPVF neurons and vRN are suppressed and excited by noxious stimuli, respectively. We combined optogenetics with calcium imaging and pharmacology to demonstrate that stimulation of NPVF cells suppresses neuronal activity in vRN. During noxious stimuli, serotonergic neurons activation was due to a suppression of an inhibitory NPVF-ventral raphe peptidergic projection. This study reveals a novel NPVF-vRN functional circuit modulated by noxious stimuli in vertebrates.

PMID:
28139691
PMCID:
PMC5282529
DOI:
10.1038/srep41528
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center