Format

Send to

Choose Destination
Food Chem Toxicol. 2017 Apr;102:46-52. doi: 10.1016/j.fct.2017.01.024. Epub 2017 Jan 28.

Anti-inflammatory effect of Capuli cherry against LPS-induced cytotoxic damage in RAW 264.7 macrophages.

Author information

1
Escuela de Medicina Veterinaria y Zootecnia, Unidad de Investigación en Biotecnología y Medio Ambiente (BIOMA), Universidad de Las Américas (UDLA), Quito, Ecuador. Electronic address: jose.alvarez@udla.edu.ec.
2
Estación Experimental Santo Domingo-Instituto Nacional de Investigaciones Agropecuarias (EESD-INIAP), Santo Domingo de los Tsáchilas, Ecuador.
3
Dipartimento di Science Cliniche Specialistiche ed Odontostomatologiche (DISCO), Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
4
Unidad de Investigación en Biotecnología y Medio Ambiente (BIOMA), Universidad de Las Américas (UDLA), Quito, Ecuador.
5
Colegio de Administración y Economía, Universidad San Francisco de Quito (USFQ), Quito, Ecuador.
6
Dipartimento di Science Cliniche Specialistiche ed Odontostomatologiche (DISCO), Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy; Centre for Nutrition & Health, Universidad Europea del Atlántico, Santander, Spain. Electronic address: m.a.battino@univpm.it.

Abstract

Capuli cherry (Prunus serotina Ehr. subsp. capuli (Cav.) McVaugh) fruits from the inter-Andean region of Ecuador were analysed to determine their bioactive compounds content, total antioxidant capacity, radical scavenging activity and their anti-inflammatory and protective effects against the cytotoxic damage mediated by lipopolysaccharide (LPS) in RAW 264.7 macrophages. Capuli fruits proved to be a natural source of bioactive compounds such as anthocyanins, vitamin C and β-carotene as well as to present an important total antioxidant capacity and radical scavenging activities. RAW 264.7 macrophages were incubated with different concentration of Capuli crude extract and subsequently activated by LPS to determine the markers related to oxidative damage and the proinflammatory cytokine production. The markers of oxidative damage, nitrite levels, the interleukin 1β messenger RNA levels and the tumor necrosis factor α mRNA levels and secretion were significantly decreased after the pre-incubated with Capuli extract and subsequently stimulated with LPS. In summary, Capuli extract attenuated the LPS-induced damage in RAW 264.7 macrophages due to its antioxidant and anti-inflammatory properties, showing that Capuli fruits may represent a relevant source of bioactive compounds with promising benefits for human health.

KEYWORDS:

Antioxidant capacity; Capuli cherry; Inflammation; Oxidative stress; Prunus serotina

PMID:
28137607
DOI:
10.1016/j.fct.2017.01.024
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center