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BMC Mol Biol. 2017 Jan 31;18(1):2. doi: 10.1186/s12867-017-0082-3.

Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5.

Author information

1
School of Public Health, Wuhan University of Science and Technology, Wuhan, 430065, Hubei, China. zhangling@wust.edu.cn.
2
Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA, 23298, USA. zhangling@wust.edu.cn.
3
School of Public Health, Wuhan University of Science and Technology, Wuhan, 430065, Hubei, China.
4
Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA, 23298, USA.
5
Central China Normal University, Wuhan, Hubei, 430000, China.
6
Department of Computer Science, Wellesley College, Wellesley, MA, 02481-5701, USA.
7
Wuhan Hospital for the Prevention and Treatment of Occupational Diseases, Wuhan, 430000, Hubei, China.
8
Department of Obstetrics and Gynecology, Virginia Commonwealth University, Richmond, VA, 23298, USA. zhibing.zhang@vcuhealth.org.

Abstract

BACKGROUND:

The mammalian sperm-associated antigen 16 gene (Spag16) uses alternative promoters to produce two major transcript isoforms (Spag16L and Spag16S) and encode proteins that are involved in the cilia/flagella formation and motility. In silico analysis of both mouse and human SPAG16L promoters reveals the existence of multiple putative SOX5 binding sites. Given that the SOX5 gene encodes a 48-kDa transcription factor (S-SOX5) and the presence of putative SOX5 binding sites at the SPAG16L promoter, regulation of SPAG16L expression by S-SOX5 was studied in the present work.

RESULTS:

S-SOX5 activated human SPAG16L promoter activity in the human bronchial epithelia cell line BEAS-2B cells. Mutation of S-SOX5 binding sites abolished the stimulatory effect. Overexpression of S-SOX5 resulted in a significant increase in the abundance of SPAG16L transcripts whereas silencing of S-SOX5 by RNAi largely reduced the SPAG16L expression. Chromatin immunoprecipitation assays showed that S-SOX5 directly interacts with the SPAG16L promoter.

CONCLUSION:

S-SOX5 regulates transcription of human SPAG16L gene via directly binding to the promoter of SPAG16L. It has been reported that expression of sperm-associated antigen 6 (SPAG6), encoding another axonemal protein, is activated by S-SOX5. Therefore, S-SOX5 may regulate formation of motile cilia/flagella through globally mediating expression of genes encoding axonemal proteins.

KEYWORDS:

Central apparatus; Cilia; S-SOX5; SPAG16L; Transcriptional regulation

PMID:
28137312
PMCID:
PMC5282894
DOI:
10.1186/s12867-017-0082-3
[Indexed for MEDLINE]
Free PMC Article

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