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Forensic Sci Int Genet. 2017 May;28:21-34. doi: 10.1016/j.fsigen.2017.01.005. Epub 2017 Jan 12.

Validation of a rapid DNA process with the RapidHIT® ID system using GlobalFiler® Express chemistry, a platform optimized for decentralized testing environments.

Author information

1
IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA.
2
IntegenX Inc, 5720 Stoneridge Drive, Suite 300, Pleasanton, CA, 94588, USA. Electronic address: davidk@integenx.com.

Abstract

The RapidHIT® ID is a fully automated sample-to-answer system for short tandem repeat (STR)-based human identification. The RapidHIT ID has been optimized for use in decentralized environments and processes presumed single source DNA samples, generating Combined DNA Index System (CODIS)-compatible DNA profiles in less than 90min. The system is easy to use, requiring less than one minute of hands-on time. Profiles are reviewed using centralized linking software, RapidLINK™ (IntegenX, Pleasanton, CA), a software tool designed to collate DNA profiles from single or multiple RapidHIT ID systems at different geographic locations. The RapidHIT ID has been designed to employ GlobalFiler® Express and AmpFLSTR® NGMSElect™, Thermo Fisher Scientific (Waltham, MA) STR chemistries. The Developmental Validation studies were performed using GlobalFiler® Express with single source reference samples according to Scientific Working Group for DNA Analysis Methods guidelines. These results show that multiple RapidHIT ID systems networked with RapidLINK software form a highly reliable system for wide-scale deployment in locations such as police booking stations and border crossings enabling real-time testing of arrestees, potential human trafficking victims, and other instances where rapid turnaround is essential.

KEYWORDS:

GlobalFiler(®) Express; Presumed single source sample; Rapid DNA RapidHIT(®); RapidHIT(®) ID; RapidLINK™; STR; SWGDAM developmental validation

PMID:
28135583
DOI:
10.1016/j.fsigen.2017.01.005
[Indexed for MEDLINE]
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