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Nat Methods. 2017 Mar;14(3):259-262. doi: 10.1038/nmeth.4153. Epub 2017 Jan 30.

Building ProteomeTools based on a complete synthetic human proteome.

Author information

1
Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany.
2
JPT Peptide Technologies GmbH, Berlin, Germany.
3
Thermo Fisher Scientific, Bremen, Germany.
4
Thermo Fisher Scientific, San Jose, California, USA.
5
SAP SE, Potsdam, Germany.
6
SAP SE, Walldorf, Germany.
7
Institute for Systems Biology, Seattle, Washington, USA.
8
Department of Biology, Institute of Molecular Systems Biology, ETH, Zürich, Switzerland.
9
Faculty of Science, University of Zürich, Zürich, Switzerland.
10
Center for Integrated Protein Science Munich, Freising, Germany.
11
Bavarian Center for Biomolecular Mass Spectrometry, Freising, Germany.

Abstract

We describe ProteomeTools, a project building molecular and digital tools from the human proteome to facilitate biomedical research. Here we report the generation and multimodal liquid chromatography-tandem mass spectrometry analysis of >330,000 synthetic tryptic peptides representing essentially all canonical human gene products, and we exemplify the utility of these data in several applications. The resource (available at http://www.proteometools.org) will be extended to >1 million peptides, and all data will be shared with the community via ProteomicsDB and ProteomeXchange.

PMID:
28135259
PMCID:
PMC5868332
DOI:
10.1038/nmeth.4153
[Indexed for MEDLINE]
Free PMC Article

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