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Molecules. 2017 Jan 27;22(2). pii: E205. doi: 10.3390/molecules22020205.

Identification of Larvicidal Constituents of the  Essential Oil of Echinops grijsii Roots against the  Three Species of Mosquitoes.

Author information

1
Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China. zhaomeiping@ioz.ac.cn.
2
Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China. lqzwyz@cau.edu.cn.
3
State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center of Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. liuqiyong@icdc.cn.
4
Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China. zhilongliu@cau.edu.cn.

Abstract

The screening of Chinese medicinal herbs for insecticidal principles showed that the essential oil of Echinops grijsii Hance roots possessed significant larvicidal activity against mosquitoes. The essential oil was extracted via hydrodistillation and its constituents were determined by gas chromatography-mass spectrometry (GC-MS) analysis. GC-MS analyses revealed the presence of 31 components, with 5-(3-buten-1-yn-1-yl)-2,2'-bithiophene (5-BBT, 27.63%), αterthienyl (α-T, 14.95%),1,8-cineole (5.56%) and cis-β-ocimene (5.01%) being the four major constituents. Based bioactivity-directed chromatographic separation of the essential oil led to the isolation of 5-BBT, 5-(4-isovaleroyloxybut-1-ynyl)-2,2'-bithiophene (5-IBT) and αT as active compounds. The essential oil of E. grijsii exhibited larvicidal activity against the fourth instar larvae of Aedes albopictus, Anopheles sinensis and Culex pipiens pallens with LC50 values of 2.65 μg/mL, 3.43 μg/mL and 1.47 μg/mL, respectively. The isolated thiophenes, 5-BBT and 5-IBT, possessed strong larvicidal activity against the fourth instar larvae of Ae. albopictus(LC50 = 0.34 μg/mL and 0.45 μg/mL, respectively) and An. sinensis(LC50 = 1.36 μg/mL and 5.36 μg/mL, respectively). The two isolated thiophenes also had LC50 values against the fourth instar larvae of C. pipiens pallens of 0.12 μg/mL and 0.33 μg/mL, respectively. The findings indicated that the essential oil of E. grijsii roots and the isolated thiophenes have an excellent potential for use in the control of Ae.albopictus, An. sinensis and C. pipiens pallens larvae and could be used in the search for new, safer and more effective natural compounds as larvicides.

KEYWORDS:

Echinops grijsii; larvicidal  activity;  Aedes albopictus;  Anopheles sinensis;  Culex pipiens pallens

PMID:
28134799
PMCID:
PMC6155871
DOI:
10.3390/molecules22020205
[Indexed for MEDLINE]
Free PMC Article

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