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Environ Sci Pollut Res Int. 2017 Mar;24(9):8017-8032. doi: 10.1007/s11356-017-8490-y. Epub 2017 Jan 29.

Looking for phosphate-accumulating bacteria in activated sludge processes: a multidisciplinary approach.

Author information

1
Microbial Processes and Interactions, TERRA Research Centre, Gembloux Agro-Bio Tech, University of Liege, Passage des Déportés 2, B-5030, Gembloux, Belgium. cedric.tarayre@ulg.ac.be.
2
Microbial Processes and Interactions, TERRA Research Centre, Gembloux Agro-Bio Tech, University of Liege, Passage des Déportés 2, B-5030, Gembloux, Belgium.
3
Entomologie Fonctionnelle et Évolutive, AgroBioChem, Gembloux Agro-Bio Tech, University of Liege, Passage des Déportés 2, B-5030, Gembloux, Belgium.
4
Chimie Analytique, AgroBioChem, Gembloux Agro-Bio Tech, University of Liege, Passage des Déportés 2, B-5030, Gembloux, Belgium.
5
Microbiologie des Denrées alimentaires, Fundamental and Applied Research for Animals and Health, University of Liege, Quartier Vallée 2, Avenue de Cureghem 10, B-4000, Liege, Belgium.
6
Département de Biologie, Ecologie et Evolution, Université de Liège, Allée du Six Août 15, B-4000, Liège, Belgium.
7
Department of Applied Analytical and Physical Chemistry, Laboratory of Analytical Chemistry and Applied Ecochemistry, Ghent University, Coupure Links 653, B-9000, Ghent, Belgium.

Abstract

Over the past decades, an increasing need in renewable resources has progressively appeared. This trend concerns not only fossil fuels but also mineral resources. Wastewater and sewage sludge contain significant concentrations in phosphate and can be considered as a fertilizer source of the utmost importance. In wastewater treatment plants, the biological uptake of phosphate is performed by a specific microbiota: the phosphate-accumulating organisms. These microorganisms are recovered in sewage sludge. Here, we aimed to investigate the occurrence of phosphate accumulators in four wastewater treatment plants. A 16S metagenetic analysis identified the main bacterial phyla extracted from the aerobic treatment: α-Proteobacteria, β-Proteobacteria, and Sphingobacteria. An enrichment stage was performed to stimulate the specific growth of phosphate-accumulating bacteria in an acetate medium. An analysis of metabolic activities of sulfur and phosphorus highlighted strong modifications related to phosphorus and much less distinguishable effects with sulfur. A solid acetate medium containing 5-Br-4-Cl-3-indolyl phosphate was used to select potential phosphate-accumulating bacteria from the enriched consortia. The positive strains have been found to belong in the genera Acinetobacter, Corynebacterium, and Pseudomonas. Finally, electron microscopy was applied to the strains and allowed to confirm the presence of polyphosphate granules. Some of these bacteria contained granules the size of which exceeded 100 nm.

KEYWORDS:

Microbial consortium; PAOs; Phosphorus recovery; Polyphosphate; Recycling; Wastewater

PMID:
28132192
DOI:
10.1007/s11356-017-8490-y
[Indexed for MEDLINE]

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