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Microb Pathog. 2017 Mar;104:202-211. doi: 10.1016/j.micpath.2017.01.042. Epub 2017 Jan 25.

Identification and initial characterisation of a protein involved in Campylobacter jejuni cell shape.

Author information

1
Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, UK.
2
Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, UK.
3
Department of Infection Biology, Institute for Infection and Global Health and School of Veterinary Science, University of Liverpool, Leahurst Campus, Neston, Cheshire, UK.
4
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
5
Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK.
6
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK; The London School of Hygiene and Tropical Medicine, London, UK.
7
Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, UK. Electronic address: ajg60@cam.ac.uk.

Abstract

Campylobacter jejuni is the leading cause of bacterial food borne illness. While helical cell shape is considered important for C. jejuni pathogenesis, this bacterium is capable of adopting other morphologies. To better understand how helical-shaped C. jejuni maintain their shape and thus any associated colonisation, pathogenicity or other advantage, it is first important to identify the genes and proteins involved. So far, two peptidoglycan modifying enzymes Pgp1 and Pgp2 have been shown to be required for C. jejuni helical cell shape. We performed a visual screen of ∼2000 transposon mutants of C. jejuni for cell shape mutants. Whole genome sequence data of the mutants with altered cell shape, directed mutants, wild type stocks and isolated helical and rod-shaped 'wild type' C. jejuni, identified a number of different mutations in pgp1 and pgp2, which result in a change in helical to rod bacterial cell shape. We also identified an isolate with a loss of curvature. In this study, we have identified the genomic change in this isolate, and found that targeted deletion of the gene with the change resulted in bacteria with loss of curvature. Helical cell shape was restored by supplying the gene in trans. We examined the effect of loss of the gene on bacterial motility, adhesion and invasion of tissue culture cells and chicken colonisation, as well as the effect on the muropeptide profile of the peptidoglycan sacculus. Our work identifies another factor involved in helical cell shape.

KEYWORDS:

Campylobacter jejuni; Cell shape

PMID:
28131954
PMCID:
PMC5335918
DOI:
10.1016/j.micpath.2017.01.042
[Indexed for MEDLINE]
Free PMC Article

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