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Microb Pathog. 2017 May;106:25-29. doi: 10.1016/j.micpath.2017.01.044. Epub 2017 Jan 25.

TGF-β1 promotes Staphylococcus aureus adhesion to and invasion into bovine mammary fibroblasts via the ERK pathway.

Author information

1
College of Animal Science, Jilin University, Changchun, China.
2
College of Veterinary Medicine, Jilin University, Changchun, China.
3
Department of Respiratory Medicine, The Second Hospital of Jilin University, Changchun, China.
4
College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address: leiliancheng@163.com.

Abstract

Fibroblasts are the structural base of mammary breast tissues. TGF-β1 can regulate the fibrotic process; however, it remains unclear whether TGF-β1 influences the susceptibility of fibroblasts to bacteria. Staphylococcus aureus (S. aureus) is a major bacterium in both chronic and subclinical mastitis in lactating cows that acts by invading host cells. To better understand the function of TGF-β1 in bovine mammary fibroblasts' (BMFBs) susceptibility to bacteria as well as the mechanisms involved, a primary BMFB model was established by treating cells with TGF-β1 followed by infection with S. aureus. The results revealed that the adhesion and invasion of S. aureus into BMFBs was significantly increased after cells were treated with 5 ng/ml TGF-β1 for 12 h. Moreover, TGF-β1 can increase Collagen I and α-SMA expression via activation of ERK signaling. However, the increased adhesion and invasion of S. aureus can be blocked by specific antibodies against either Collagen I or α-SMA, indicating that the increased adhesion and invasion are dependent on TGF-β1-induced upregulation of both Collagen I and α-SMA. Using PD98059, an ERK inhibitor, could also decrease the adhesion and invasion of S. aureus. These results indicate that TGF-β1 could promote S. aureus adhesion to and invasion into BMFBs by increasing Collagen I and α-SMA expression and may provide a novel target for controlling bovine mastitis.

KEYWORDS:

Bovine mammary fibroblast; ERK; Staphylococcus aureus; TGF-β1

PMID:
28131949
DOI:
10.1016/j.micpath.2017.01.044
[Indexed for MEDLINE]

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