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Genes Dev. 2017 Jan 1;31(1):34-45. doi: 10.1101/gad.289728.116.

The PIDDosome activates p53 in response to supernumerary centrosomes.

Author information

1
Division of Developmental Immunology, Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria.
2
Department of Internal Medicine VI, Infectious Diseases, Immunology, Rheumatology, Pneumology, Medical University of Innsbruck, 6020 Innsbruck, Austria.
3
Division of Molecular Pathophysiology, Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria.
4
Biozentrum, University of Basel, 4056 Basel, Switzerland.
5
Tyrolean Cancer Research Institute, 6020 Innsbruck, Austria.

Abstract

Centrosomes, the main microtubule-organizing centers in animal cells, are replicated exactly once during the cell division cycle to form the poles of the mitotic spindle. Supernumerary centrosomes can lead to aberrant cell division and have been causally linked to chromosomal instability and cancer. Here, we report that an increase in the number of mature centrosomes, generated by disrupting cytokinesis or forcing centrosome overduplication, triggers the activation of the PIDDosome multiprotein complex, leading to Caspase-2-mediated MDM2 cleavage, p53 stabilization, and p21-dependent cell cycle arrest. This pathway also restrains the extent of developmentally scheduled polyploidization by regulating p53 levels in hepatocytes during liver organogenesis. Taken together, the PIDDosome acts as a first barrier, engaging p53 to halt the proliferation of cells carrying more than one mature centrosome to maintain genome integrity.

KEYWORDS:

cell division; centrosome; cytokinesis failure; p53

Comment in

PMID:
28130345
PMCID:
PMC5287111
DOI:
10.1101/gad.289728.116
[Indexed for MEDLINE]
Free PMC Article

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