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Clin Chim Acta. 2017 Mar;466:167-171. doi: 10.1016/j.cca.2017.01.025. Epub 2017 Jan 24.

Using circular RNA hsa_circ_0000190 as a new biomarker in the diagnosis of gastric cancer.

Author information

1
Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo 315211, China.
2
Department of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo 315211, China. Electronic address: guojunming@nbu.edu.cn.

Abstract

BACKGROUND:

Circular RNAs (circRNA) are an abundant class of non-coding RNAs in mammalian cells. However, their value in the diagnosis of cancers remains unknown. In this study, we focused on hsa_circ_0000190, which was found to be down-regulated in gastric cancer tissues in our previous microarray screening.

PATIENTS AND METHODS:

The hsa_circ_0000190 levels in 104 paired gastric cancer tissues and adjacent non-tumor tissues, 104 plasma samples from patients with gastric cancer and 104 plasma samples from health controls were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Then, the association between the expression level of hsa_circ_0000190 and the clinicopathological features of patients with gastric cancer was further analyzed. A receiver operating characteristic (ROC) curve was generated to evaluate the diagnostic value.

RESULTS:

Hsa_circ_0000190 was first found to be down-regulated in gastric cancer tissues (P<0.001) and plasma samples from patients with gastric cancer (P<0.001). Its expression levels were significantly correlated with tumor diameter (P=0.034), lymphatic metastasis (P=0.026), distal metastasis (P=0.001), TNM stage (P=0.001), and CA19-9 levels (P=0.019). The areas under the ROC curve (AUC) of hsa_circ_0000190 in tissues and plasma were up to 0.75 and 0.60, respectively. The sensitivity and specificity of the combination were 0.712 and 0.750; the AUC was increased to 0.775.

CONCLUSIONS:

These results indicated that hsa_circ_0000190 may be a novel non-invasive biomarker for the diagnosis of gastric cancer. Its AUC, sensitivity and specificity are better than commonly used biomarkers such as CEA and CA19-9.

KEYWORDS:

Biomarker; Circular RNA; Gastric cancer; Hsa_circ_0000190

PMID:
28130019
DOI:
10.1016/j.cca.2017.01.025
[Indexed for MEDLINE]

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