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Nat Commun. 2017 Jan 27;8:14242. doi: 10.1038/ncomms14242.

A Lin28 homologue reprograms differentiated cells to stem cells in the moss Physcomitrella patens.

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National Institute for Basic Biology, Division of Evolutionary Biology, Okazaki 444-8585, Japan.
Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Japan.
ERATO, Hasebe Reprogramming Evolution Project, Japan Science and Technology Agency, Okazaki 444-8585, Japan.
Advanced Science Research Center, Institute for Gene Research, Kanazawa University, Kanazawa 920-0934, Japan.
Division of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia 26506, USA.
Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan.
Department of Biology and Howard Hughes Medical Institute, Duke University, Durham, North Carolina 27708, USA.


Both land plants and metazoa have the capacity to reprogram differentiated cells to stem cells. Here we show that the moss Physcomitrella patens Cold-Shock Domain Protein 1 (PpCSP1) regulates reprogramming of differentiated leaf cells to chloronema apical stem cells and shares conserved domains with the induced pluripotent stem cell factor Lin28 in mammals. PpCSP1 accumulates in the reprogramming cells and is maintained throughout the reprogramming process and in the resultant stem cells. Expression of PpCSP1 is negatively regulated by its 3'-untranslated region (3'-UTR). Removal of the 3'-UTR stabilizes PpCSP1 transcripts, results in accumulation of PpCSP1 protein and enhances reprogramming. A quadruple deletion mutant of PpCSP1 and three closely related PpCSP genes exhibits attenuated reprogramming indicating that the PpCSP genes function redundantly in cellular reprogramming. Taken together, these data demonstrate a positive role of PpCSP1 in reprogramming, which is similar to the function of mammalian Lin28.

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