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Development. 2017 Mar 1;144(5):820-829. doi: 10.1242/dev.136713. Epub 2017 Jan 26.

Neuroblast niche position is controlled by Phosphoinositide 3-kinase-dependent DE-Cadherin adhesion.

Author information

1
Department of Biology, University of Virginia, Charlottesville, VA 22903, USA.
2
Department of Biology, University of Virginia, Charlottesville, VA 22903, USA ses4gr@virginia.edu.

Abstract

Correct positioning of stem cells within their niche is essential for tissue morphogenesis and homeostasis. How stem cells acquire and maintain niche position remains largely unknown. Here, we show that a subset of brain neuroblasts (NBs) in Drosophila utilize Phosphoinositide 3-kinase (PI3-kinase) and DE-cadherin to build adhesive contact for NB niche positioning. NBs remain within their native microenvironment when levels of PI3-kinase activity and DE-cadherin are elevated in NBs. This occurs through PI3-kinase-dependent regulation of DE-Cadherin-mediated cell adhesion between NBs and neighboring cortex glia, and between NBs and their ganglion mother cell daughters. When levels of PI3-kinase activity and/or DE-Cadherin are reduced in NBs, NBs lose niche position and relocate to a non-native brain region that is rich in neurosecretory neurons, including those that secrete some of the Drosophila insulin-like peptides. Linking levels of PI3-kinase activity to the strength of adhesive attachment could provide cancer stem cells and hematopoietic stem cells with a means to cycle from trophic-poor to trophic-rich microenvironments.

KEYWORDS:

DE-Cadherin; Drosophila; Neuroblast; Niche; PI3-kinase; Shotgun; Stem cell

PMID:
28126840
PMCID:
PMC5374343
DOI:
10.1242/dev.136713
[Indexed for MEDLINE]
Free PMC Article

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