Format

Send to

Choose Destination
FASEB J. 2017 May;31(5):1964-1975. doi: 10.1096/fj.201601127R. Epub 2017 Jan 26.

Premature remodeling of fat body and fat mobilization triggered by platelet-derived growth factor/VEGF receptor in Drosophila.

Zheng H1,2,3,4, Wang X4, Guo P1,2,3, Ge W1,2,3, Yan Q4, Gao W5,6, Xi Y7,2,3, Yang X8,2,3,9.

Author information

1
Division of Human Reproduction and Developmental Genetics, The Women's Hospital, and.
2
Department of Genetics, Zhejiang University School of Medicine, Hangzhou, China.
3
Institute of Genetics and.
4
College of Life Sciences, Zhejiang University, Hangzhou, China.
5
School of Biomedical Engineering and.
6
Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China; and.
7
Division of Human Reproduction and Developmental Genetics, The Women's Hospital, and xyyongm@zju.edu.cn.
8
Division of Human Reproduction and Developmental Genetics, The Women's Hospital, and xhyang@zju.edu.cn.
9
Joint Institute of Genetics and Genomic Medicine, Zhejiang University-University of Toronto, Zhejiang University, Hangzhou, China.

Abstract

In Drosophila, fat-body remodeling accompanied with fat mobilization is an ecdysone-induced dynamic process that only occurs during metamorphosis. Here, we show that the activated Drosophila platelet-derived growth factor/VEGF receptor (PVR) is sufficient to induce shape changes in the fat body, from thin layers of tightly conjugated polygonal cells to clusters of disaggregated round-shaped cells. These morphologic changes are reminiscent of those seen during early pupation upon initiation of fat-body remodeling. Activation of PVR also triggers an early onset of lipolysis and mobilization of internal storage, as revealed by the appearance of small lipid droplets and up-regulated lipolysis-related genes. We found that PVR displays a dynamic expression pattern in the fat body and peaks at the larval-prepupal transition under the control of ecdysone signaling. Removal of PVR, although it does not prevent ecdysone-induced fat-body remodeling, causes ecdysone signaling to be up-regulated. Our data reveal that PVR is active in a dual-secured mechanism that involves an ecdysone-induced fat-body remodeling pathway and a reinforced PVR pathway for effective lipid mobilization. Ectopic expression of activated c-kit-the mouse homolog of PVR in the Drosophila fat body-also results in a similar phenotype. This may suggest a novel function of c-kit as it relates to lipid metabolism in mammals.-Zheng, H., Wang, X., Guo, P., Ge, W., Yan, Q., Gao, W., Xi, Y., Yang, X. Premature remodeling of fat body and fat mobilization triggered by platelet-derived growth factor/VEGF receptor in Drosophila.

KEYWORDS:

CD117/c-kit; Rac1; ecdysone; fat cell; lipid metabolism

PMID:
28126734
DOI:
10.1096/fj.201601127R
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center