Format

Send to

Choose Destination
Am J Physiol Gastrointest Liver Physiol. 2017 Mar 1;312(3):G257-G265. doi: 10.1152/ajpgi.00410.2016. Epub 2017 Jan 26.

CRISPR/Cas 9 genome editing and its applications in organoids.

Author information

1
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), Utrecht, The Netherlands.
2
University Medical Center (UMC) Utrecht, Utrecht, The Netherlands; and.
3
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), Utrecht, The Netherlands; h.clevers@hubrecht.eu.
4
Princess Maxima Center, Utrecht, The Netherlands.

Abstract

Organoids are three-dimensional (3D) structures derived from adult or embryonic stem cells that maintain many structural and functional features of their respective organ. Recently, genome editing based on the bacterial defense mechanism CRISPR/Cas9 has emerged as an easily applicable and reliable laboratory tool. Combining organoids and CRISPR/Cas9 creates exciting new opportunities to study organ development and human disease in vitro. The potential applications of CRISPR in organoids are only beginning to be explored.

KEYWORDS:

CRISPR/Cas9; gene editing; organoid

PMID:
28126704
DOI:
10.1152/ajpgi.00410.2016
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center