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Virology. 2017 Mar;503:52-61. doi: 10.1016/j.virol.2017.01.009. Epub 2017 Jan 23.

Characterization of contrasting features between hepatitis B virus genotype A and genotype D in small envelope protein expression and surface antigen secretion.

Author information

1
Key Lab of Medical Molecular Virology, School of Basic Medical Sciences, Fudan University, Shanghai, China.
2
Liver Research Center, Rhode Island Hospital, Warren Alpert School of Medicine, Brown University, Providence, RI, USA.
3
Division of Gastroenterology and Hepatology, University of Michigan, Ann Arbor, MI, USA.
4
Department of Infectious Diseases, Huashan Hospital, Fudan University, Shanghai, China.
5
Key Lab of Medical Molecular Virology, School of Basic Medical Sciences, Fudan University, Shanghai, China; Liver Research Center, Rhode Island Hospital, Warren Alpert School of Medicine, Brown University, Providence, RI, USA. Electronic address: shuping_tong_md@brown.edu.

Abstract

Hepatitis B virus (HBV) genotypes A and D are prevalent in many parts of the world and show overlapping geographic distributions. We amplified the entire HBV genome from sera of patients with genotypes A and D and generated overlength constructs for transient transfection into Huh7 or HepG2 cells. Genotype D clones were associated with less HBsAg in culture supernatant and even less intracellular HBsAg. They produced less 2.1-kb RNA due to a weaker SPII promoter. Chimeric promoter constructs identified three divergent positions as most critical, and their exchange reversed extracellular HBsAg phenotype. The S protein of genotype D was more efficient at secretion, while its L protein possessed greater inhibitory effect. Swapping the S gene diminished genotypic difference in intracellular S protein but widened the difference in secreted HBsAg. In conclusion, HBV genotypes A and D differ in S protein expression, secretion and modulation by L protein.

KEYWORDS:

Genotype; Hepatitis B surface antigen; Hepatitis B virus; Large envelope protein; SPII promoter; Small envelope protein

PMID:
28126637
PMCID:
PMC5325793
DOI:
10.1016/j.virol.2017.01.009
[Indexed for MEDLINE]
Free PMC Article

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