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PLoS One. 2017 Jan 26;12(1):e0168814. doi: 10.1371/journal.pone.0168814. eCollection 2017.

Crystal Structure of an Invasivity-Associated Domain of SdrE in S. aureus.

Author information

1
Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, PR China.
2
Department of Laboratory Medicine, People's Hospital of YuBei District, YuBei District, Chongqing, People's Republic of China.
3
Department of Laboratory Medicine, Chongqing Medical University, YixueYuanlu-1, Chongqing, People's Republic of China.
4
Department of Laboratory Medicine, Hospital of Zhejiang, Xihu District, Hangzhou, People's Republic of China.
5
School of Life Science, the University of Science and Technology of China, Hefei, Anhui, People's Republic of China.

Abstract

The surface protein SdrE, a microbial surface components recognizing adhesive matrix molecule (MSCRAMM) family protein expressed on the surface of Staphylococcus aureus (S. aureus), can recognize human complement regulator Factor H and C4BP, thus making it a potentially promising vaccine candidate. In this study, SdrE278-591 was found to directly affect S. aureus host cell invasion. Additionally, the crystal structure of SdrE278-591 at a resolution of 1.25 Å was established, with the three-dimensional structure revealing N2-N3 domains which fold in a manner similar to an IgG fold. Furthermore, a putative ligand binding site located at a conserved charged groove formed by the interface between N2 and N3 domains was identified, with β2 suspected to occupy the ligand recognizing site and undergo a structural rearrangement to allow ligand binding. Overall, these findings have further contributed to the understanding of SdrE as a key factor for S. aureus invasivity and will enable a better understanding of bacterial infection processes.

PMID:
28125581
PMCID:
PMC5268492
DOI:
10.1371/journal.pone.0168814
[Indexed for MEDLINE]
Free PMC Article

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