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Angew Chem Int Ed Engl. 2017 Feb 20;56(9):2356-2360. doi: 10.1002/anie.201610926. Epub 2017 Jan 26.

Dual Fluorescent- and Isotopic-Labelled Self-Assembling Vancomycin for in vivo Imaging of Bacterial Infections.

Author information

1
Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, P.R. China.
2
Department of Chemistry, Brandeis University, Waltham, MA, 02454, USA.
3
State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, P.R. China.

Abstract

The increase of bacterial resistance demands rapid and accurate diagnosis of bacterial infections. Biosurface-induced supramolecular assembly for diagnosis and therapy has received little attention in detecting bacterial infections. Herein we present a dual fluorescent-nuclear probe based on self-assembly of vancomycin (Van) on Gram-positive bacteria for imaging bacterial infection. A Van- and rhodamine-modified peptide derivative (Rho-FF-Van), as the imaging agent, binds to the terminal peptide of the methicillin-resistant staphylococcus aureus (MRSA) and self-assembles to form nanoaggregates on the surface of MRSA. In an in vivo myositis model, Rho-FF-Van results in a significant increased fluorescence signal at the MRSA infected site. Radiolabeled with iodine-125, Rho-FF-Van shows strong radioactive signal in the MRSA-infected lungs in a murine model. This novel dual fluorescent and nuclear probe promises a new way for in vivo imaging of bacterial infections.

KEYWORDS:

bacterial infections; fluorescent probes; in vivo imaging; isotopic labeling; self-assembly; vancomycin

PMID:
28124815
DOI:
10.1002/anie.201610926
[Indexed for MEDLINE]

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