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Open Biol. 2017 Jan;7(1). pii: 160258. doi: 10.1098/rsob.160258.

TGFβ/Activin signalling is required for ribosome biogenesis and cell growth in Drosophila salivary glands.

Author information

1
I3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4150-180, Portugal tjn29@cam.ac.uk.
2
Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, Porto 4150-180, Portugal.
3
Cell Cycle Development Group, Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.
4
I3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4150-180, Portugal.
5
Centro Andaluz de Biología del Desarrollo (CABD), CSIC-JA-Universidad Pablo de Olavide. Ctra. de Utrera km1, Seville 41013, Spain.
6
I3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto 4150-180, Portugal paulop@ibmc.up.pt.

Abstract

Signalling by TGFβ superfamily factors plays an important role in tissue growth and cell proliferation. In Drosophila, the activity of the TGFβ/Activin signalling branch has been linked to the regulation of cell growth and proliferation, but the cellular and molecular basis for these functions are not fully understood. In this study, we show that both the RII receptor Punt (Put) and the R-Smad Smad2 are strongly required for cell and tissue growth. Knocking down the expression of Put or Smad2 in salivary glands causes alterations in nucleolar structure and functions. Cells with decreased TGFβ/Activin signalling accumulate intermediate pre-rRNA transcripts containing internal transcribed spacer 1 regions accompanied by the nucleolar retention of ribosomal proteins. Thus, our results show that TGFβ/Activin signalling is required for ribosomal biogenesis, a key aspect of cellular growth control. Importantly, overexpression of Put enhanced cell growth induced by Drosophila Myc, a well-characterized inducer of nucleolar hypertrophy and ribosome biogenesis.

KEYWORDS:

Drosophila; Punt (Put); TGFβ/Activin; cell growth; nucleolus; ribosome

PMID:
28123053
PMCID:
PMC5303274
DOI:
10.1098/rsob.160258
[Indexed for MEDLINE]
Free PMC Article

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