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J Neurophysiol. 2017 Apr 1;117(4):1608-1614. doi: 10.1152/jn.00939.2016. Epub 2017 Jan 25.

Optimal delineation of single C-tactile and C-nociceptive afferents in humans by latency slowing.

Author information

1
Department of Physiology, University of Gothenburg, Gothenburg, Sweden; roger.holmes.watkins@gu.se.
2
School of Physiology and Pharmacology, University of Bristol, Bristol, United Kingdom.
3
Department of Physiology, University of Gothenburg, Gothenburg, Sweden.
4
University Division of Anaesthesia, Cambridge University Hospitals, NHS Foundation Trust, Cambridge, United Kingdom.
5
Center for Social and Affective Neuroscience, Department of Clinical and Experimental Medicine, Linkoping University, Linkoping, Sweden; and.
6
Department of Physiological Sciences, University of Florida, Gainesville, Florida.
7
Laboratoire de Neurosciences Intégratives et Adaptatives (UMR 7260), Aix-Marseille Université-CNRS, Marseille, France.

Abstract

C-mechanoreceptors in humans comprise a population of unmyelinated afferents exhibiting a wide range of mechanical sensitivities. C-mechanoreceptors are putatively divided into those signaling gentle touch (C-tactile afferents, CTs) and nociception (C-mechanosensitive nociceptors, CMs), giving rise to positive and negative affect, respectively. We sought to distinguish, compare, and contrast the properties of a population of human C-mechanoreceptors to see how fundamental the divisions between these putative subpopulations are. We used microneurography to record from individual afferents in humans and applied electrical and mechanical stimulation to their receptive fields. We show that C-mechanoreceptors can be distinguished unequivocally into two putative populations, comprising CTs and CMs, by electrically evoked spike latency changes (slowing). After both natural mechanical stimulation and repetitive electrical stimulation there was markedly less latency slowing in CTs compared with CMs. Electrical receptive field stimulation, which bypasses the receptor end organ, was most effective in classifying C-mechanoreceptors, as responses to mechanical receptive field stimulation overlapped somewhat, which may lead to misclassification. Furthermore, we report a subclass of low-threshold CM responding to gentle mechanical stimulation and a potential subclass of CT afferent displaying burst firing. We show that substantial differences exist in the mechanisms governing axonal conduction between CTs and CMs. We provide clear electrophysiological "signatures" (extent of latency slowing) that can be used in unequivocally identifying populations of C-mechanoreceptors in single-unit and multiunit microneurography studies and in translational animal research into affective touch. Additionally, these differential mechanisms may be pharmacologically targetable for separate modulation of positive and negative affective touch information.NEW & NOTEWORTHY Human skin encodes a plethora of touch interactions, and affective tactile information is primarily signaled by slowly conducting C-mechanoreceptive afferents. We show that electrical stimulation of low-threshold C-tactile afferents produces markedly different patterns of activity compared with high-threshold C-mechanoreceptive nociceptors, although the populations overlap in their responses to mechanical stimulation. This fundamental distinction demonstrates a divergence in affective touch signaling from the first stage of sensory processing, having implications for the processing of interpersonal touch.

KEYWORDS:

C fiber; human; low-threshold mechanoreceptor; microneurography; nociceptor

PMID:
28123010
PMCID:
PMC5376601
DOI:
10.1152/jn.00939.2016
[Indexed for MEDLINE]
Free PMC Article

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