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Mol Cell Proteomics. 2017 Apr;16(4):524-536. doi: 10.1074/mcp.M116.062414. Epub 2017 Jan 25.

Unlocking Cancer Glycomes from Histopathological Formalin-fixed and Paraffin-embedded (FFPE) Tissue Microdissections.

Author information

1
From the ‡Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, 14424 Potsdam, Germany.
2
§Freie Universität Berlin, Department of Biology, Chemistry, Pharmacy, Institute of Chemistry and Biochemistry, 14195 Berlin, Germany.
3
¶Faculty of Science, Department of Biology, Division of Molecular Biology, University of Zagreb, Zagreb, Croatia.
4
‖Institute for Pathology and Cytology, University Hospital Merkur, Zagreb, Croatia.
5
**Department of Pathology, Medical School Zagreb, University of Zagreb, Zagreb, Croatia.
6
From the ‡Max Planck Institute of Colloids and Interfaces, Department of Biomolecular Systems, 14424 Potsdam, Germany; daniel.kolarich@mpikg.mpg.de.

Abstract

N- and O-glycans are attractive clinical biomarkers as glycosylation changes in response to diseases. The limited availability of defined clinical specimens impedes glyco-biomarker identification and validation in large patient cohorts. Formalin-fixed paraffin-embedded (FFPE) clinical specimens are the common form of sample preservation in clinical pathology, but qualitative and quantitative N- and O-glycomics of such samples has not been feasible to date. Here, we report a highly sensitive and glycan isomer selective method for simultaneous N- and O-glycomics from histopathological slides. As few as 2000 cells isolated from FFPE tissue sections by laser capture microdissection were sufficient for in-depth histopathology-glycomics using porous graphitized carbon nanoLC ESI-MS/MS. N- and O-glycan profiles were similar between unstained and hematoxylin and eosin stained FFPE samples but differed slightly compared with fresh tissue. This method provides the key to unlock glyco-biomarker information from FFPE histopathological tissues archived in pathology laboratories worldwide.

PMID:
28122943
PMCID:
PMC5383776
DOI:
10.1074/mcp.M116.062414
[Indexed for MEDLINE]
Free PMC Article

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