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J Cell Sci. 2017 Mar 1;130(5):975-988. doi: 10.1242/jcs.197343. Epub 2017 Jan 25.

IKKα regulates human keratinocyte migration through surveillance of the redox environment.

Author information

1
Davis Center for Regenerative Biology and Medicine, MDI Biological Laboratory, 159 Old Bar Harbor Road, Salisbury Cove, ME 04672, USA srieger@mdibl.org thomas.lisse@jax.org.
2
The Jackson Laboratory, Bar Harbor, ME 04609, USA.

Abstract

Although the functions of H2O2 in epidermal wound repair are conserved throughout evolution, the underlying signaling mechanisms are largely unknown. In this study we used human keratinocytes (HEK001) to investigate H2O2-dependent wound repair mechanisms. Scratch wounding led to H2O2 production in two or three cell layers at the wound margin within ∼30 min and subsequent cysteine modification of proteins via sulfenylation. Intriguingly, exogenous H2O2 treatment resulted in preferential sulfenylation of keratinocytes that adopted a migratory phenotype and detached from neighboring cells, suggesting that one of the primary functions of H2O2 is to stimulate signaling factors involved in cell migration. Based on previous findings that revealed epidermal growth factor receptor (EGFR) involvement in H2O2-dependent cell migration, we analyzed oxidation of a candidate upstream target, the inhibitor of κB kinase α (IKKα; encoded by CHUK), as a mechanism of action. We show that IKKα is sulfenylated at a conserved cysteine residue in the kinase domain, which correlates with de-repression of EGF promoter activity and increased EGF expression. Thus, this indicates that IKKα promotes migration through dynamic interactions with the EGF promoter depending on the redox state within cells.

KEYWORDS:

EGF; H2O2; HEK001; Hydrogen peroxide; IKKα; Keratinocytes; Migration; Nuclear de-repression; Oxidation; Reactive oxygen species; Sulfenylation; Wound healing; Wound repair

PMID:
28122935
PMCID:
PMC5358334
DOI:
10.1242/jcs.197343
[Indexed for MEDLINE]
Free PMC Article

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