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J Clin Microbiol. 2017 Apr;55(4):1154-1161. doi: 10.1128/JCM.02354-16. Epub 2017 Jan 25.

Is Follow-Up Testing with the FilmArray Gastrointestinal Multiplex PCR Panel Necessary?

Author information

1
Department of Laboratory Medicine, Ewha Womans University School of Medicine, Seoul, South Korea.
2
Clinical Microbiology Laboratory, Stanford Health Care, Stanford, California, USA.
3
Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA.
4
Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.
5
Department of Pathology, Stanford University School of Medicine, Stanford, California, USA nbanaei@stanford.edu.

Abstract

The FilmArray gastrointestinal (GI) panel (BioFire Diagnostics, Salt Lake City, UT) is a simple, sample-to-answer, on-demand, multiplex, nucleic acid amplification test for syndromic diagnosis of infectious gastroenteritis. The aim of this study was to measure the yield of follow-up testing with FilmArray GI panel within 4 weeks of an initial test. Consecutive adult and pediatric patients tested at an academic institution between August 2015 and June 2016 were included in this study. Of 145 follow-up tests in 106 unique patients with an initial negative result, 134 (92.4%) tests and 98 (92.5%) patients remained negative upon follow-up testing. Excluding targets that are not reported at this institution (Clostridium difficile, enteroaggregative Escherichia coli, enteropathogenic E. coli, and enterotoxigenic E. coli), 137 (94.5%) follow-up tests and 101 (95.3%) patients remained negative. Weekly conversion rates were not significantly different across the 4-week follow-up interval. No epidemiological or clinical factors were significantly associated with a negative to positive conversion. Of 80 follow-up tests in patients with an initial positive result, 43 (53.8%) remained positive for the same target, 34 (42.5%) were negative, and 3 were positive for a different target (3.8%). Follow-up testing with FilmArray GI panel within 4 weeks of a negative result rarely changed the initial result, and the follow-up test reverted to negative less than half the time after an initial positive result. In the absence of clinical or epidemiological evidence for a new infection, follow-up testing should be limited and FilmArray GI panel should not be used as a test of cure.

KEYWORDS:

FilmArray gastrointestinal; GI PCR; repeat; syndromic

PMID:
28122874
PMCID:
PMC5377843
DOI:
10.1128/JCM.02354-16
[Indexed for MEDLINE]
Free PMC Article

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