Format

Send to

Choose Destination
Cell Rep. 2017 Jan 24;18(4):1033-1047. doi: 10.1016/j.celrep.2016.12.044.

Cilia Control Vascular Mural Cell Recruitment in Vertebrates.

Author information

1
Vesalius Research Center, VIB-KUL, Leuven 3000, Belgium.
2
Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Turin, Turin 10126, Italy.
3
Vesalius Research Center, VIB-KUL, Leuven 3000, Belgium; Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Turin, Turin 10126, Italy. Electronic address: massimo.santoro@kuleuven.vib.be.

Abstract

Vascular mural cells (vMCs) are essential components of the vertebrate vascular system, controlling blood vessel maturation and homeostasis. Discrete molecular mechanisms have been associated with vMC development and differentiation. The function of hemodynamic forces in controlling vMC recruitment is unclear. Using transgenic lines marking developing vMCs in zebrafish embryos, we find that vMCs are recruited by arterial-fated vessels and that the process is flow dependent. We take advantage of tissue-specific CRISPR gene targeting to demonstrate that hemodynamic-dependent Notch activation and the ensuing arterial genetic program is driven by endothelial primary cilia. We also identify zebrafish foxc1b as a cilia-dependent Notch-specific target that is required within endothelial cells to drive vMC recruitment. In summary, we have identified a hemodynamic-dependent mechanism in the developing vasculature that controls vMC recruitment.

KEYWORDS:

CRISPR-Cas9; blood flow; cilia; mural cells; zebrafish model

PMID:
28122229
PMCID:
PMC5289940
DOI:
10.1016/j.celrep.2016.12.044
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center