Send to

Choose Destination
J Histochem Cytochem. 2017 Apr;65(4):207-221. doi: 10.1369/0022155416689670. Epub 2017 Jan 25.

Histochemical Examination on Periodontal Tissues of Klotho-Deficient Mice Fed With Phosphate-Insufficient Diet.

Author information

Department of Developmental Biology of Hard Tissue (KH, TH, ET, HH, TY, AK, MH, NA), Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
Department of Orthodontics (KH, JI), Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan.
Department of Applied Prosthodontics, Unit of Translational Medicine, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan (MS).
Division of Biochemistry, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan (KO).
Department of Oral and Maxillofacial Surgery, Dr. Mário Gatti Municipal Hospital, Campinas, Brazil (PHLF).
Shandong Provincial Key Laboratory of Oral Biomedicine, School of Stomatology, Shandong University, Jinan, China (ML).


To elucidate which of elevated serum concentration of inorganic phosphate (Pi) or disrupted signaling linked to αklotho/fibroblast growth factor 23 (FGF23) is a predominant regulator for senescence-related degeneration seen in αKlotho-deficient mice, we have examined histological alteration of the periodontal tissues in the mandibular interalveolar septum of αKlotho-deficient mice fed with Pi-insufficient diet. We prepared six groups of mice: wild-type, kl/kl, and αKlotho-/- mice with normal diet or low-Pi diet. As a consequence, kl/klnorPi and αKlotho-/-norPi mice showed the same abnormalities in periodontal tissues: intensely stained areas with hematoxylin in the interalveolar septum, dispersed localization of alkaline phosphatase-positive osteoblasts and tartrate-resistant acid phosphatase-reactive osteoclasts, and accumulation of dentin matrix protein 1 in the osteocytic lacunae. Although kl/kllowPi mice improved these histological abnormalities, αKlotho-/- lowPi mice failed to normalize those. Gene expression of αKlotho was shown to be increased in kl/kl lowPi specimens. It seems likely that histological abnormalities of kl/kl mice have been improved by the rescued expression of αKlotho, rather than low concentration of serum Pi. Thus, the histological malformation in periodontal tissues in αKlotho-deficient mice appears to be due to not only increased concentration of Pi but also disrupted αklotho/FGF23 signaling.


DMP-1; osteopontin; periodontal tissue; phosphate; αKlotho-deficient mice

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center