Format

Send to

Choose Destination
Nature. 1989 Nov 16;342(6247):243-7.

Scanning from an independently specified branch point defines the 3' splice site of mammalian introns.

Author information

1
Howard Hughes Medical Institute, Department of Cardiology, Children's Hospital, Boston, Massachusetts.

Abstract

During pre-messenger RNA splicing the lariat branch point in mammalian introns is specified independently of the 3' splice site by the sequence surrounding the branch point and by an adjacent downstream polypyrimidine tract. The 3' splice site is dispensable for spliceosome assembly and cleavage at the 5' splice site, and is itself determined by a scanning process that recognizes the first AG located 3' of the branch point/polypyrimidine tract, irrespective of distance or sequence environment.

PMID:
2812024
DOI:
10.1038/342243a0
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center