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J Chromatogr A. 2017 Feb 24;1486:35-41. doi: 10.1016/j.chroma.2017.01.040. Epub 2017 Jan 18.

Recommendations for quantitative analysis of small molecules by matrix-assisted laser desorption ionization mass spectrometry.

Author information

1
Department of Pharmaceutical Sciences and Barnett Institute, Bouve College, Northeastern University, Boston, MA 02115, USA.
2
Department of Pharmaceutical Sciences and Barnett Institute, Bouve College, Northeastern University, Boston, MA 02115, USA. Electronic address: r.giese@northeastern.edu.

Abstract

Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) has been used for quantitative analysis of small molecules for many years. It is usually preceded by an LC separation step when complex samples are tested. With the development several years ago of "modern MALDI" (automation, high repetition laser, high resolution peaks), the ease of use and performance of MALDI as a quantitative technique greatly increased. This review focuses on practical aspects of modern MALDI for quantitation of small molecules conducted in an ordinary way (no special reagents, devices or techniques for the spotting step of MALDI), and includes our ordinary, preferred methods The review is organized as 18 recommendations with accompanying explanations, criticisms and exceptions.

KEYWORDS:

MALDI-MS; Ordinary conditions; Quantitative analysis; Recommendations; Sample spotting; Small molecules

PMID:
28118972
PMCID:
PMC5323262
DOI:
10.1016/j.chroma.2017.01.040
[Indexed for MEDLINE]
Free PMC Article

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