Format

Send to

Choose Destination
J Am Chem Soc. 2017 Feb 8;139(5):1810-1813. doi: 10.1021/jacs.6b13148. Epub 2017 Jan 24.

Enhanced Nonenzymatic RNA Copying with 2-Aminoimidazole Activated Nucleotides.

Author information

1
Howard Hughes Medical Institute, Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital , Boston, Massachusetts 02114, United States.

Abstract

Achieving efficient nonenzymatic replication of RNA is an important step toward the synthesis of self-replicating protocells that may mimic early forms of life. Despite recent progress, the nonenzymatic copying of templates containing mixed sequences remains slow and inefficient. Here we demonstrate that activating nucleotides with 2-aminoimidazole results in superior reaction kinetics and improved yields of primer extension reaction products. This new leaving group significantly accelerates monomer addition as well as trimer-assisted RNA primer extension, allowing efficient copying of a variety of short RNA templates with mixed sequences.

PMID:
28117989
PMCID:
PMC6326525
DOI:
10.1021/jacs.6b13148
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for American Chemical Society Icon for PubMed Central
Loading ...
Support Center