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Sci Pharm. 2016 Apr 13;84(3):555-566. doi: 10.3390/scipharm84030555.

Metabolite Profiles in Various Plant Organs of Justicia gendarussa Burm.f. and Its in Vitro Cultures.

Author information

1
Faculty of Pharmacy, Airlangga University, Jl. Dharmawangsa Dalam, Surabaya 60286, Indonesia. indrayoniputu@gmail.com.
2
PT. Angler BioChemLab, Plaza Graha Family C-25, Surabaya 60226, Indonesia. intan@anglerlab.net.
3
PT. Angler BioChemLab, Plaza Graha Family C-25, Surabaya 60226, Indonesia. dir@anglerlab.net.
4
Faculty of Pharmacy, Airlangga University, Jl. Dharmawangsa Dalam, Surabaya 60286, Indonesia. prajogo_ew@yahoo.com.
5
Faculty of Pharmacy, Airlangga University, Jl. Dharmawangsa Dalam, Surabaya 60286, Indonesia. gunawanindrayanto@yahoo.com.

Abstract

Metabolite profiles of plant organs and their in vitro cultures of Justicia gendarussa have been studied by using Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight-Mass Spectrometry (UPLC-Qtof-MS). Samples of leaves, stems, roots, and shoot cultures showed similar patterns of metabolites, while samples of root cultures gave very different profiles. Concentrations of secondary metabolites in shoot cultures were relatively low compared to those in the leaves and roots of the plants. The results suggested that secondary metabolites in J. gendarussa were biosynthetized in the leaves, then transported to the roots.

KEYWORDS:

Justicia gendarussa Burm.f.; PCA; UPLC-Qtof-MS; in vitro cultures; plant organs; secondary metabolites accumulation

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