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J Biol Chem. 2017 Mar 3;292(9):3877-3887. doi: 10.1074/jbc.M116.762245. Epub 2017 Jan 23.

P3h3-null and Sc65-null Mice Phenocopy the Collagen Lysine Under-hydroxylation and Cross-linking Abnormality of Ehlers-Danlos Syndrome Type VIA.

Author information

1
From the Department of Orthopaedics and Sports Medicine, University of Washington, Seattle, Washington 98195, dmhudson@uw.edu.
2
From the Department of Orthopaedics and Sports Medicine, University of Washington, Seattle, Washington 98195.
3
the Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, and.
4
the Department of Physiology & Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205.

Abstract

Tandem mass spectrometry was applied to tissues from targeted mutant mouse models to explore the collagen substrate specificities of individual members of the prolyl 3-hydroxylase (P3H) gene family. Previous studies revealed that P3h1 preferentially 3-hydroxylates proline at a single site in collagen type I chains, whereas P3h2 is responsible for 3-hydroxylating multiple proline sites in collagen types I, II, IV, and V. In screening for collagen substrate sites for the remaining members of the vertebrate P3H family, P3h3 and Sc65 knock-out mice revealed a common lysine under-hydroxylation effect at helical domain cross-linking sites in skin, bone, tendon, aorta, and cornea. No effect on prolyl 3-hydroxylation was evident on screening the spectrum of known 3-hydroxyproline sites from all major tissue collagen types. However, collagen type I extracted from both Sc65-/- and P3h3-/- skin revealed the same abnormal chain pattern on SDS-PAGE with an overabundance of a γ112 cross-linked trimer. The latter proved to be from native molecules that had intramolecular aldol cross-links at each end. The lysine under-hydroxylation was shown to alter the divalent aldimine cross-link chemistry of mutant skin collagen. Furthermore, the ratio of mature HP/LP cross-links in bone of both P3h3-/- and Sc65-/- mice was reversed compared with wild type, consistent with the level of lysine under-hydroxylation seen in individual chains at cross-linking sites. The effect on cross-linking lysines was quantitatively very similar to that previously observed in EDS VIA human and Plod1-/- mouse tissues, suggesting that P3H3 and/or SC65 mutations may cause as yet undefined EDS variants.

KEYWORDS:

bone; collagen; cross-links; endoplasmic reticulum (ER); mass spectrometry (MS); post-translational modification (PTM); skin

PMID:
28115524
PMCID:
PMC5339768
DOI:
10.1074/jbc.M116.762245
[Indexed for MEDLINE]
Free PMC Article

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