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Circ Cardiovasc Genet. 2017 Jan;10(1). pii: e001534. doi: 10.1161/CIRCGENETICS.116.001534.

The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle.

Author information

1
From the Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital (L.A.S., M.I.S., T.-W.C., M.H., S.K.S., S.C.B., J.D.M.), Division of Cardiac Surgery, Department of Surgery, Brigham and Women's Hospital (S.F.A.), and Department of Genetics (E.M., C.E.S., J.G.S.), Harvard Medical School, Boston, MA.
2
From the Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital (L.A.S., M.I.S., T.-W.C., M.H., S.K.S., S.C.B., J.D.M.), Division of Cardiac Surgery, Department of Surgery, Brigham and Women's Hospital (S.F.A.), and Department of Genetics (E.M., C.E.S., J.G.S.), Harvard Medical School, Boston, MA. jmuehlschlegel@partners.org.

Abstract

BACKGROUND:

The discovery of functional classes of long noncoding RNAs (lncRNAs) has expanded our understanding of the variety of RNA species that exist in cells. In the heart, lncRNAs have been implicated in the regulation of development, ischemic and dilated cardiomyopathy, and myocardial infarction. Nevertheless, there is a limited description of expression profiles for these transcripts in human subjects.

METHODS AND RESULTS:

We obtained left ventricular tissue from human patients undergoing cardiac surgery and used RNA sequencing to describe an lncRNA profile. We then identified a list of lncRNAs that were differentially expressed between pairs of samples before and after the ischemic insult of cardiopulmonary bypass. The expression of some of these lncRNAs correlates with ischemic time. Coding genes in close proximity to differentially expressed lncRNAs and coding genes that have coordinated expression with these lncRNAs are enriched in functional categories related to myocardial infarction, including heart function, metabolism, the stress response, and the immune system.

CONCLUSIONS:

We describe a list of lncRNAs that are differentially expressed after ischemia in the human heart. These genes are predicted to function in pathways consistent with myocardial injury. As a result, lncRNAs may serve as novel diagnostic and therapeutic targets for ischemic heart disease.

CLINICAL TRIAL REGISTRATION:

URL: http://www.clinicaltrials.gov. Unique identifier: NCT00985049.

KEYWORDS:

aortic valve; myocardial infarction; myocardial ischemia; nucleotides; transcriptional activation

PMID:
28115490
PMCID:
PMC5302288
DOI:
10.1161/CIRCGENETICS.116.001534
[Indexed for MEDLINE]
Free PMC Article

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