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Nat Methods. 2017 Mar;14(3):271-274. doi: 10.1038/nmeth.4148. Epub 2017 Jan 23.

Optogenetic inhibition of behavior with anion channelrhodopsins.

Author information

1
Program in Neuroscience and Behavioral Disorders, Duke-NUS Medical School, Singapore.
2
Institute for Molecular and Cell Biology, Agency for Science Technology and Research, Singapore.
3
Temasek Life Sciences Laboratory, Singapore.
4
Department of Physiology, National University of Singapore, Singapore.

Abstract

Optogenetics uses light exposure to manipulate physiology in genetically modified organisms. Abundant tools for optogenetic excitation are available, but the limitations of current optogenetic inhibitors present an obstacle to demonstrating the necessity of neuronal circuits. Here we show that anion channelrhodopsins can be used to specifically and rapidly inhibit neural systems involved in Drosophila locomotion, wing expansion, memory retrieval and gustation, thus demonstrating their broad utility in the circuit analysis of behavior.

PMID:
28114289
DOI:
10.1038/nmeth.4148
[Indexed for MEDLINE]

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