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DNA Cell Biol. 2017 Feb;36(2):177-187. doi: 10.1089/dna.2016.3471.

Photoprotective Potential of Glycolic Acid by Reducing NLRC4 and AIM2 Inflammasome Complex Proteins in UVB Radiation-Induced Normal Human Epidermal Keratinocytes and Mice.

Hung SJ1,2, Tang SC2,3,4, Liao PY1,2, Ge JS1, Hsiao YP5,6, Yang JH1,3,7.

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1 Department of Dermatology, Buddhist Tzu Chi General Hospital , Hualien, Taiwan .
2 Institute of Medical Sciences, Tzu Chi University , Hualien, Taiwan .
3 Department of Biochemistry, School of Medicine, Tzu Chi University , Hualien, Taiwan .
4 Institute of Biomedical Sciences , Academia Sinica, Taipei, Taiwan .
5 Institute of Medicine, School of Medicine, Chung Shan Medical University , Taichung, Taiwan .
6 Department of Dermatology, Chung Shan Medical University Hospital , Taichung, Taiwan .
7 Institute of Medicine, School of Medicine, Tzu Chi University , Hualien, Taiwan .


Exposure to UVB radiation induces inflammation and free radical-mediated oxidative stress through reactive oxygen species (ROS) that play a crucial role in the induction of skin cancer. Glycolic acid (GA) is frequently used in cosmetics and dermatology. The aim of the study was to analyze the photoprotective mechanisms through which GA retards UVB-induced ROS accumulation and inflammation in normal human epidermal keratinocytes (NHEKs) and mice skin, respectively. NHEK cell line and C57BL/6J mice were treated with GA (0.1 or 5 mM) for 24 h followed by UVB irradiation. ROS accumulation, DNA damage, and expression of inflammasome complexes (NLRP3, NLRC4, ASC, and AIM2) were measured in vitro. Epidermal thickness and inflammasome complex proteins were analyzed in vivo. GA significantly prevented UVB-induced loss of skin cell viability, ROS formation, and DNA damage (single and double strands DNA break). GA suppressed the mRNA expression levels of NLRC4 and AIM2 among the inflammasome complexes. GA also blocked interleukin (IL)-1β by reducing the activity of caspase-1 in the NHEKs. Treatment with GA (2%) inhibited UVB-induced inflammation marker NLRC4 protein levels in mouse dorsal skin. The photoprotective activity of GA was ascribed to the inhibition of ROS formation and DNA damage, as well as a reduction in the activities of inflammasome complexes and IL-1β. We propose that GA has anti-inflammatory and photoprotective effects against UVB irradiation. GA is potentially beneficial to the protection of human skin from UV damage.


glycolic acid–alpha-hydroxy acids; inflammasome complexes–NLRC4; normal human epidermal keratinocytes; reactive oxygen species

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