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Int J Biochem Cell Biol. 2017 Mar;84:89-95. doi: 10.1016/j.biocel.2017.01.004. Epub 2017 Jan 20.

Characterising live cell behaviour: Traditional label-free and quantitative phase imaging approaches.

Author information

1
Phasefocus Ltd, Sheffield, UK; Technology Facility, University of York, UK. Electronic address: richard.kasprowicz@york.ac.uk.
2
Phasefocus Ltd, Sheffield, UK; Technology Facility, University of York, UK.
3
Technology Facility, University of York, UK. Electronic address: peter.otoole@york.ac.uk.

Abstract

Label-free imaging uses inherent contrast mechanisms within cells to create image contrast without introducing dyes/labels, which may confound results. Quantitative phase imaging is label-free and offers higher content and contrast compared to traditional techniques. High-contrast images facilitate generation of individual cell metrics via more robust segmentation and tracking, enabling formation of a label-free dynamic phenotype describing cell-to-cell heterogeneity and temporal changes. Compared to population-level averages, individual cell-level dynamic phenotypes have greater power to differentiate between cellular responses to treatments, which has clinical relevance e.g. in the treatment of cancer. Furthermore, as the data is obtained label-free, the same cells can be used for further assays or expansion, of potential benefit for the fields of regenerative and personalised medicine.

KEYWORDS:

Label-free imaging; Live-cell imaging; Ptychography; QPI; Quantitative phase imaging

PMID:
28111333
DOI:
10.1016/j.biocel.2017.01.004
[Indexed for MEDLINE]
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