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Malar J. 2017 Jan 21;16(1):37. doi: 10.1186/s12936-017-1697-z.

Development of Plasmodium falciparum specific naïve, atypical, memory and plasma B cells during infancy and in adults in an endemic area.

Author information

1
School of Biomedical Sciences, College of Health Sciences, Makerere University, Kampala, Uganda.
2
Department of Microbiology, Tumor, and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
3
Habib Medical School, Islamic University in Uganda (IUIU), Kampala, Uganda.
4
Department of Microbiology, Tumor, and Cell Biology, Karolinska Institutet, Stockholm, Sweden. kristina.persson@med.lu.se.
5
Department of Laboratory Medicine, Lund University, Lund, Sweden. kristina.persson@med.lu.se.

Abstract

BACKGROUND:

B-cells are essential in immunity against malaria, but which sub-sets of B-cells specifically recognize Plasmodium falciparum and when they appear is still largely unknown.

RESULTS:

Using the flow cytometry technique for detection of P. falciparum specific (Pf+) B-cells, this study for the first time measured the development of Pf+ B cell (CD19+) phenotypes in Ugandan babies from birth up to nine months, and in their mothers. The babies showed increases in Pf+ IgG memory B-cells (MBCs), atypical MBCs, and plasma cells/blasts over time, but the proportion of these cells were still lower than in the mothers who displayed stable levels (5, 18, and 3%, respectively). Pf+ non-IgG+ MBCs and naïve B-cells binding to P. falciparum antigens were higher in the babies compared to the mothers (12 and 50%). In ELISA there was an increase in IgG and IgM antibodies over time in babies, and stable levels in mothers. At baby delivery, multigravidae mothers had a higher proportion of Pf+ IgG MBCs and less Pf+ naïve B-cells than primigravidae mothers.

CONCLUSIONS:

In newborns, naïve B-cells are a major player in recognizing P. falciparum. In adults, the high proportion of Pf+ atypical MBCs suggests a major role for these cells. Both in infants and adults, non-IgG+ MBCs were higher than IgG MBCs, indicating that these cells deserve more focus in future.

KEYWORDS:

Atypical; B-cells; Immunity; Malaria; Memory; Plasmodium falciparum

PMID:
28109284
PMCID:
PMC5251336
DOI:
10.1186/s12936-017-1697-z
[Indexed for MEDLINE]
Free PMC Article

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