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Int J Syst Evol Microbiol. 2017 May;67(5):1296-1305. doi: 10.1099/ijsem.0.001800. Epub 2017 Jun 5.

Nostoc thermotolerans sp. nov., a soil-dwelling species of Nostoc (Cyanobacteria).

Author information

1
1​Microbial Culture Collection (MCC), National Centre for Cell Science (NCCS), Pune, India.
2
2​Department of Biology, University of North Florida, Jacksonville, FL 32224, USA.
3
3​Department of Bioecology and Biological Education, M. Akmullah Bashkir State Pedagogical University, Ufa 450000, Russian Federation 4​All-Russian Research Institute of Phytopathology, Moscow Region, Odintsovo district, B.Vyazyomy, Institute street, 5, Russian Federation.

Abstract

A filamentous, soil-dwelling cyanobacterial strain (9C-PST) was isolated from Mandsaur, Madhya Pradesh, India, and is described as a new species of the genus Nostoc. Extensive morphological and molecular characterization along with a thorough assessment of ecology was performed. The style of filament orientation, type and nature of the sheath (e.g. distribution and visibility across the trichome), and vegetative and heterocyte cell dimensions and shape were assessed for over one year using both the laboratory grown culture and the naturally occurring samples. Sequencing of the 16S rRNA gene showed 94 % similarity with Nostocpiscinale CENA21 while analyses of the secondary structures of the 16S-23S ITS region showed unique folding patterns that differentiated this strain from other species of Nostoc. The level of rbcl and rpoC1 gene sequence similarity was 91 and 94 % to Nostocsp. PCC 7524 and Nostocpiscinale CENA21, respectively, while the nifD gene sequence similarity was found to be 99 % with Nostocpiscinale CENA21. The phenotypic, ecological, genetic and phylogenetic observations indicate that the strain 9C-PST represents a novel species of the genus Nostoc with the name proposed being Nostoc thermotolerans sp. nov. according to the International Code of Nomenclature for Algae, Fungi, and Plants.

PMID:
28109209
DOI:
10.1099/ijsem.0.001800
[Indexed for MEDLINE]

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