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Enferm Infecc Microbiol Clin. 2017 May;35(5):303-313. doi: 10.1016/j.eimc.2016.12.010. Epub 2017 Jan 17.

Fast methods of fungal and bacterial identification. MALDI-TOF mass spectrometry, chromogenic media.

[Article in English, Spanish]

Author information

1
Instituto de Investigación Biomédica de Salamanca (IBSAL), Complejo Asistencial Universitario de Salamanca, Universidad de Salamanca, CSIC, Salamanca, España.
2
Instituto de Investigación Biomédica de Salamanca (IBSAL), Complejo Asistencial Universitario de Salamanca, Universidad de Salamanca, CSIC, Salamanca, España; Servicio de Bioquímica Clínica, Complejo Asistencial Universitario de Salamanca, Salamanca, España; Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, Salamanca, España.
3
Instituto de Investigación Biomédica de Salamanca (IBSAL), Complejo Asistencial Universitario de Salamanca, Universidad de Salamanca, CSIC, Salamanca, España; Servicio de Microbiología, Complejo Asistencial Universitario de Salamanca, Salamanca, España; Departamento de Ciencias Biomédicas y del Diagnóstico, Universidad de Salamanca, Salamanca, España. Electronic address: jlmubel@usal.es.

Abstract

MALDI-TOF mass spectrometry is now a routine resource in Clinical Microbiology, because of its speed and reliability in the identification of microorganisms. Its performance in the identification of bacteria and yeasts is perfectly contrasted. The identification of mycobacteria and moulds is more complex, due to the heterogeneity of spectra within each species. The methodology is somewhat more complex, and expanding the size of species libraries, and the number of spectra of each species, will be crucial to achieve greater efficiency. Direct identification from blood cultures has been implemented, since its contribution to the management of severe patients is evident, but its application to other samples is more complex. Chromogenic media have also contributed to the rapid diagnosis in both bacteria and yeast, since they accelerate the diagnosis, facilitate the detection of mixed cultures and allow rapid diagnosis of resistant species.

KEYWORDS:

Chromogenic media; Espectrometría de masas MALDI-TOF; Identificación; Identification; MALDI-TOF mass spectrometry; Medios cromogénicos

PMID:
28108122
DOI:
10.1016/j.eimc.2016.12.010
[Indexed for MEDLINE]

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