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Anal Chim Acta. 2017 Mar 8;957:63-69. doi: 10.1016/j.aca.2016.12.024. Epub 2016 Dec 27.

One-step synthesis of DNA functionalized cadmium-free quantum dots and its application in FRET-based protein sensing.

Author information

1
Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China. Electronic address: clzhang@chem.ecnu.edu.cn.
2
Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.
3
School of Chemistry and Chemical Engineering, Lingnan Normal University, Zhanjiang, 524048, China.
4
Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China. Electronic address: yzxian@chem.ecnu.edu.cn.

Abstract

DNA functionalized quantum dots (QDs) are promising nanoprobes for the fluorescence resonance energy transfer (FRET)-based biosensing. Herein, cadmium-free DNA functionalized Mn-doped ZnS (DNA-ZnS:Mn2+) QDs were successfully synthesized by one-step route. As-synthesized QDs show excellent photo-stability with the help of PAA and DNA. Then, we constructed a novel FRET model based on the QDs and WS2 nanosheets as the energy donor-acceptor pairs, which was successfully applied for the protein detection through the terminal protection of small molecule-linked DNA assay. This work not only explores the potential bioapplication of the DNA-ZnS:Mn2+ QDs, but also provides a platform for the investigation of small molecule-protein interaction.

KEYWORDS:

Biosensor; FRET; Quantum dots; Terminal protection of small-molecule linked DNA; WS(2)

PMID:
28107835
DOI:
10.1016/j.aca.2016.12.024
[Indexed for MEDLINE]

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