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J Am Chem Soc. 2017 Feb 8;139(5):1766-1769. doi: 10.1021/jacs.6b12180. Epub 2017 Jan 25.

2'-O-Methyl-5-hydroxymethylcytidine: A Second Oxidative Derivative of 5-Methylcytidine in RNA.

Author information

1
Department of Chemistry, University of Cambridge , Lensfield Road, Cambridge CB2 1EW, United Kingdom.
2
Wellcome Trust/Cancer Research UK Gurdon Institute , Tennis Court Road, Cambridge CB2 1QN, United Kingdom.

Abstract

5-Hydroxymethylcytidine (hm5C) was recently identified as a direct metabolite of m5C in RNA. We investigated the stability of hm5C in human cells using bio-isotopologues and LC-MS/HRMS. This has led to the discovery of a second oxidative metabolite of m5C in RNA, namely 2'-O-methyl-5-hydroxymethylcytidine (hm5Cm). Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels and TET-independent formation of this new RNA modification.

PMID:
28107630
PMCID:
PMC5504488
DOI:
10.1021/jacs.6b12180
[Indexed for MEDLINE]
Free PMC Article

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